DNA

Part:BBa_K3171179:Design

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-19)


HisD 600bp Homologous part 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 252
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Specific gRNA targets were selected for knockout of the HisD gene.


Source

HisD Homologous sequence

References